Phospholipase Dfrom Actinomadura sp.
Phosphatidylcholine phosphatidohydrolase EC 3.1.4.4
| Phospholipase D from Actinomadura sp. Phosphatidylcholine phosphatidohydrolase EC 3.1.4.4 |
| Phospholipase D hydrolyzes an ester bond between phosphatidic acid and choline. In the presence of alcoholic compounds, this enzyme is also capable of transferring phosphatidic acid moiety to a variety of primary or secondary alcoholic compounds.. |
| Appearance | Beige powder |
| Activity | 1,500 U/g powder |
| Molecular weight | 58,000 (SDS-PAGE) |
| Isoelectric point | 6.4 |
| Optimum pH | 5.5 |
| pH stability | 4 - 8 |
| Optimum temperature | 50 ºC |
| Thermal stability | Below 30 ºC |
| Activator | Na+, Ca2+, diethylether, Triton X-100 |
| Inhibitor | Cationic detergents |
| Storage | Desiccate and store below 25 ºC |